Improving lignocellulosic biofuel production by CRISPR/Cas9?mediated lignin modification in barley

نویسندگان

چکیده

Barley is a major cereal crop with wide ecological range, and its lignocellulosic residues have the potential to be used as feedstock for various purposes, including biofuel production. Lignocellulosic biomass an abundant renewable source of carbon energy. However, heterogeneous properties intrinsic recalcitrance caused by cell wall lignification lowered biorefinery efficiency. The reduced lignin content and/or altered structure been desirable traits feedstock. We report reduction in barley CRISPR/Cas9-mediated mutagenesis caffeic acid O-methyltransferase 1 (HvCOMT1), biosynthetic gene responsible syringyl unit formation. transgene-free, homozygous HvCOMT1 mutant was generated analyzed composition, saccharification efficiency, bioethanol production performance. had 14% lower total 34% higher fermentable glucose recovery rate, compared wild-type (WT). concentration yield from hydrolysates were 14.3 g/L 0.46 g/g sugar, respectively. This result 12% than those obtained WT (10.7 0.41 sugar). Under controlled environmental conditions, overall growth performance similar WT, no distinct morphological variations between them. could offer improved quality efficient

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ژورنال

عنوان ژورنال: Gcb Bioenergy

سال: 2021

ISSN: ['1757-1693', '1757-1707']

DOI: https://doi.org/10.1111/gcbb.12808